Purification and catalytic behavior optimization of lactose degrading β-galactosidase from Aspergillus nidulans
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چکیده
منابع مشابه
PARTIAL PURIFICATION AND CHARACTERIZATION OF B-GALACTOSIDASE FROM ASPERGILLUS NIGER UV-5
The enzyme pgalactosidase from a mutant strain of A. niger UV-5 was partially purified using ammonium sulfate and acetone. The saturation range of 60-80% ammonium sulfate was found to yield 60.5% enzyme recovery with 2.4 fold purification. Acetone precipitation at enzyme: acetone ratio of 1 : 1.5 brought about a higher yield i.e. 68% and three-fold purification. The combined procedures of ...
متن کاملPurification and characterization of phosphatase I from Aspergillus nidulans.
Repressible alkaline phosphatases have been reported to occur in a variety of microorganisms, including Es&e&& coli (1, 2), Staphylococcus aureus (3)) Bacillus subtilis (4)) Neurospora crassa (5), and baker’s yeast (6). Under conditions of limiting phosphate, the fungus Aspergillus nidulans produces substantial amounts of four electrophoretically distinct phosphomonesterases (7). Two of these e...
متن کاملPurification and Characterization of Phosphatase I from Aspergillus nidulans*
Repressible alkaline phosphatases have been reported to occur in a variety of microorganisms, including Es&e&& coli (1, 2), Staphylococcus aureus (3)) Bacillus subtilis (4)) Neurospora crassa (5), and baker’s yeast (6). Under conditions of limiting phosphate, the fungus Aspergillus nidulans produces substantial amounts of four electrophoretically distinct phosphomonesterases (7). Two of these e...
متن کاملPurification and preliminary characterization of alcohol dehydrogenase from Aspergillus nidulans.
Aspergillus alcohol dehydrogenase is produced in response to growth in the presence of a wide variety of inducers, of which the most effective are short-chain alcohols and ketones, e.g. butan-2-one and propan-2-ol. The enzyme can be readily extracted from fresh or freeze-dried cells and purified to homogeneity on Blue Sepharose in a single step by using specific elution with NAD+ and pyrazole. ...
متن کاملpartial purification and characterization of b-galactosidase from aspergillus niger uv-5
the enzyme pgalactosidase from a mutant strain of a. niger uv-5 was partially purified using ammonium sulfate and acetone. the saturation range of 60-80% ammonium sulfate was found to yield 60.5% enzyme recovery with 2.4 fold purification. acetone precipitation at enzyme: acetone ratio of 1 : 1.5 brought about a higher yield i.e. 68% and three-fold purification. the combined procedures of 1.5 v...
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ژورنال
عنوان ژورنال: Journal of Food Science and Technology
سال: 2018
ISSN: 0022-1155,0975-8402
DOI: 10.1007/s13197-018-3470-x